Ill 



hydrate would have acted much more quickly (in the 

 dilutions used about ten times) than an equivalent 

 amount of ammonia. Now this is so far from being 

 the case that, on the contrary, ammonia acts more than 

 twice (2,24) as quickly, three different experiments sho- 

 wing the proportions 1,93, 2,00 and 2,78 in the case of 

 0,01 n solution. In this concentration sodium hydrate 

 contains about 20 times as many hydroxylions as the 

 equivalent amount of ammonia. This shows clearly 

 that in this case it is not the hydroxylions which are 

 active. To the same conclusion we are also led by the 

 fact that the action of ammonia is in proportion to its 

 concentration and not to its square root, the same oc- 

 curring with sodium hydrate. Probably, in this case, 

 dissociated, as well as undissociated molecules, contribute 

 to the haemolysis. 



Most experiments in haemolysis have been made by 

 subjecting the tubes to a temperature of 37 C. for 1 

 hour and afterwards leaving them to cool in an ice 

 safe for about 20 hours. We have therefore found it well 

 to study the influence of time on experiments orranged 

 as above. As appears from the above example, the 

 velocity of reaction in the ice safe (temperature 6 7 C.) 

 is about Y 20 of what it is at 37 C. But during a few 

 hours the blood corpuscles subside and by that means 

 avoid further haemolysis. 



In the following experiments the test tubes we're 

 therefore allowed to stand a certain time in the incu- 

 bator, at 37 C. and were then put into a freezing mix- 

 ture, in which they quickly cooled down to about 0; 

 afterwards they were put in an ice safe for about 20 

 hours. The headings, given below, indicate the time 

 during which the test tubes were subjected to 37 C. 

 Total haemolysis corresponds in this case to GO. 



