Ill 



In these lables we do not find the rapid increase of 

 haemolysis in proportion to the time taken, as in the 

 preceding experiments. This depends of course on the 

 long time passed at the lower temperature, after the 

 time spent at high temperature. These experiments, 

 therefore, do not give such a clear idea of the process 

 of htemolysing as the last. But they are rather useful 

 for the right conception of the results of the usual 

 method of experimentation. The length of time devoted 

 to heating the tubes has a much greater influence on 

 the action of tetanolysin than on that of bases. And 

 between these the length of time has more influence 

 on ammonia than on sodium hydrate. This seems to 

 indicate that it is the hydrolysed part of the combina- 

 tion of the three lysins with the blood corpuscles, the 

 action of which increases with the rise of the tempera- 

 ture. Probably its hydrolysis increases with increasing 

 temperature and this, in the highest degree, is the case 

 with the combination most easily hydrolysed -- that of 

 tetanolysin, and in the smallest degree in the case of 

 the most stable combination - - that of sodium. The 

 greatest relative action of the temperature is found in 

 the case of slight concentrations of tetanolysin. 



Action of foreign bodies. 



Foreign bodies usually have an effect upon haemoly- 

 sis; in most cases the effect is weak. In some cases, 

 on the other hand, it is exceedingly strong as, for in- 

 stance, in the case of normal serum acting upon lela- 

 nolysin and in the case of salts of ammonia acting 

 upon ammonia. This action being partly of a great 

 practical significance and on the whole very important 

 for the understanding of haemolysis, we have tried to 

 find such cases, even when they were not very obvious. 



