Ill 



The action is of about same character, but at higher 

 concentrations considerably stronger than that of the 

 salts of the strong alkalies, still it is much smaller than 

 the action of ammonia salts upon the strength of am- 

 monia. In this case the largest addition of salt would 

 decrease the degree of dissociation in the case of l / S80 n 

 ammonia to about y 200 ; as may be calculated from the 

 constant of dissociation in the case of ammonia at 25 

 C (K - 23 10-). 



A dissociation constant of the size K = 2 10 -a resp. 

 4 10 3 would be required to obtain a decrease corre- 

 sponding to the above mentioned in the case of t he- 

 combination of sodium hydrate and ammonia with 

 blood corpuscles. Constants of this magnitude belong to 

 the chlorophtalic and trichlorlactic acids which are among 

 the strongest organic acids. 



The salts have also an action, though not a strong 

 one, 0/2 tetanolysin. This appears from the fact that 

 the action of tetanolysin is stronger in salt than in su- 

 gar solution. Still small doses have no perceptible ef- 

 fect, as appears from the following example. The expe- 

 riments were carried out by adding to tetanolysin sodium 

 chloride and sodium sulphate (in 0,9 and 0,2 n solu- 

 tions) and ammonium sulphate (in 0,75 n and 0,2 n so- 

 lutions). The action of the weaker doses is impercep- 

 tible, the differences being smaller than the errors of 

 experiment. The action of equivalent amounts of the 

 three salts is nearly similar and amounts to about 50% 

 in a 0,04 n solution. A salt solution of the strength 0,01 n 

 or less has no or a very little influence. The strength 

 of the lysin in the added solutions was 0,2 % (= 

 addition of 0,5 cc. 0,9 n. salt solution to 10 cc. blood 

 emulsion). 



41 



