Ill 



One must list about 50 % more lysin in order to 

 have the same effect on the "unwashed" blood. This 

 quantity may evidently be different in different cases. 



For our experiments we therefore only used blood 

 corpuscles which had been twice very thoroughly 

 w r ashed. 



As the albumen or serum added to ammonia or 

 sodium hydrate has a very different effect from what 

 it has when added to tetanolysin, we cannot assume 



that this action concerns the blood corpuscles; in that 

 case the effect ought to be the same in both cases. We 

 must therefore suppose that the serum or albumen 

 has a direct action upon the lysin or some combination 

 of it. As we hawe seen, the tetanolysin probably com- 

 bines with red blood corpuscles, and under such cir- 

 cumstances it is very likely that also the albumen or 

 serum or perhaps some of their components combine 

 with tetanolysin, which in this way becomes weakened. 

 The amount of tetanolysin combination formed is nearly 

 proportionate to the added amount of albumen or serum, 

 but only to a certain point, at which practically all 

 tetanolysin has been transformed to the weaker com- 



50 - 



