Ill 



necessary for the further progress of the reaction. The 

 solubility of this body, which would be an electrolyte, 

 would be decreased through addition of similar ions. 

 In this case il must also be supposed that the solubility 

 or the degree of dissociation of the salts of the strong 

 bases (KOH, NaOH and LiOH) were almost similar, 

 while on the contrary the solubility (or the degree of 

 dissociation) of the salt of ammonia is considerably 

 smaller. The small effect of the alkaline earths probably 

 depends on the formation of insoluble products of the 

 haemolysis, which therefore in no higher degree can 

 manifest itself. 



Hut the sails of the strong bases and of ammonia 

 have also another effect upon haemolysing processes 

 having an increasing effect upon tetanolysin. This pro- 

 bably depends on a direct action on the red blood cor- 

 puscles, which through the addition of salt are rendered, 

 so to speak, more susceptible of attack. This effect is 

 of course supraposed to that just mentioned, which in 

 consequence is apparently weakened. Still the increa- 

 sing effect of salts upon ruemolysis can be demonstra- 

 ted by the examination of the velocity of reaction. 1 cc. 

 n NaCI solution added to a solution of 0,5 cc. 0,5 n 

 NaOH in 10 cc. 2,5% blood emulsion in cane-sugar so- 

 lution, at 22,4 C increased the velocity of haemolysis by 

 about 25%: when half the amount of NaCI was added, 

 the velocity was increased by half that quantity. Sodium 

 nitrate seems in these experiments to have about the 

 same action as Nad, perhaps a little stronger: the ac- 

 tion of the sodium sulphate on the contrary is only 

 half as strong. All the ammonia salts have, on the 

 other hand, a strong decreasing effect on the velocity 

 of reaction, yet not to such a great extent as in the 



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