Ill 



p 14,55 indicates, that 1 cc. of the antitoxin used 

 Y4bo(%) is equivalent to 14 s 55 cc. of the above supposed 

 arbitrary unit of toxin. Or in other terms: the unit 

 of toxin used corresponds to 0,069 cc. of the antitoxin 

 used. 



In Fig. 4 this circumstance is represented by the 

 fact that the tangent of the G-curve in the point n == 

 cuts the abscissa in the point n 0,276, for 0,276 cc. of 

 Y40o/o antitoxin are sufficient to neutralise 2 cc. of 2% 

 lysin i.e. 4 toxin-units that are used in making the 

 toxic mixture. 



By means of the values of p and K later on the 

 values G calc. and x calc. have been calculated. The 

 concordance between the observed and calculated values 

 of x is exceedingly good. The lacking concordance, 

 which perhaps might be found in the case of the hig- 

 hest values of n, for insl. Xobs. = 12,5; Xcalc. = = 10,2 is 

 more apparent than real, for in the case of these great 

 amounts of antitoxin the equilibrium between fixed and 

 free antitoxin and toxin is obtained very slowly. It is 

 therefore necessary to permit the solutions of toxin and 

 antitoxin to be mixed for a long time, before they are used 

 for the experiments. But the tetanolysin is weakened, 

 when it is kept in solution during a longer time, and 

 any special good concordance can therefore not be ex- 

 pected in this case. All experiments with great amounts 

 at' antitoxin (from and with n - 0,50) give also a too 

 great value of Xobs. compared with Xcalc. In other terms: 

 the toxin was weaker than would be expected from the 

 experiments with small amounts of antitoxin. The 

 toxin becomes weaker through the long time of reac- 

 tion, and as a rule the more so the longer time the mix- 

 lure of toxin and antitoxin lias to be kept before it 

 is used. 







- 61 - 



