Ill 



elated salt 1 ), but from dissociated ammonia and boracic 

 acid, is minimal in comparison with those ions, arising 

 from dissociated salt. In the present case the blood isemul- 

 sionated in 0,8 % Nacl (= 0,14 n). The quantity of 

 salt, formed through combination with ammonia, never 

 amounting to 0,006 normal in the above experiments, 

 the total concentration of the different solutions may 

 be considered as subjected to only such small varia- 

 tions that the degree of dissociation of the salts keeps 

 almost completely constant. Therefore the number of 

 ammonium ions is proportionate to the amount of am- 

 monia combined, and the number of borateions pro- 

 portionate to the amount of boracic acid combined, which 

 is equivalent to the amount of ammonia combined. 



Thus we get the following equation: 



(amount of free NH 3 ) (amount of free H 3 3 B) = 

 K (amount of combined NH 3 ) a 



This is exactly the same equation as previously found in 

 the case of tetanolysin, when toxin is exchanged for am- 

 monia and antitoxin for boracic acid. In this case the 

 experiment is not so simple as in the case of lysin, the 

 newly formed ammonia salt decreasing the action of am- 

 monia. Still with these small amounts it can be con- 

 cluded without recognizable error, that when ammonia- 

 salt is added in an arithmetical progression, the hiumo- 

 lysing property of ammonia decreases in geometrical 

 progression. In the following calculation our starting 

 point was the fact, previously ascertained, that 0.01 n 

 ammoniumsalt decreases the action of ammonia to 40 

 percent. If thus the degree of haemolysis used as unit 

 is produced by 0,00167 n ammonia, as above stated, 



i) Cf. S. Arrhenius: Zeitschr. f. physic. Chemic. o i (1890). 



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