Ill 



The curves of fig. 5 are made analogous to that of 

 tetanolysin (fig. 4); an out drawn line representing the 

 values of a, with upright standing crosses indicating the 

 corresponding observed values, and a dotted line the cal- 

 culated and observed values for 1 : a. The latter corre- 

 sponds to the curve of toxicity and ought according to the 

 old nomenclature to be called the toxin spectrum of am- 

 monia. There is a striking agreement between this curve 

 and the corresponding curve for tetanolysin. They only 

 differ as regards the constants, these being different in 

 the two cases (0,117 for tetanolysin, 1,02 for ammonia). 

 How insignificant this difference is appears from the 

 fact, that tetanolysin, kept in solution for a certain 

 time, becomes weaker; this effects an increase of the 

 constant. As will later on be shown, this constant du- 

 ring the time May 1900 June 1902 had increased from 

 0.12 to 1.7 and had thus once passed the value 1.02. If 

 the toxin had been examined at this time, the curves 

 of toxicity of ammonia and tetanolysin had been iden- 

 tical. 



The consequence of all this is, that the decrease of 

 the action of tetanolysin through antitoxin in all like 

 lyhood is caused by a combination of the two bodies. 

 As so often occurs, especially in the case of organic 

 combinations, this combination is partly decomposed in 

 its components, so that a chemical equilibrium between 

 the latter and the combination takes place according to 

 the above formula (the law of Gnldberg-Waage). This 

 formula indicates that of one molecule of toxin and of 

 one molecule of antitoxin two molecules of the combi- 

 nation toxin-antitoxin are formed. 



The great likeness between the state of equilibrium 

 between ammonia, boracic acid and ammonia borale and 

 their ions, needs not go so far that it is necessary to 



