Ill 



tion was found by allowing it to continue for a long 

 lime (400 to 450 minutes); a moderate equalization is 

 applied lo these values. 



For the calculation of the amounts A and B this limit 

 was taken as point and the velocity of reaction deter- 

 mined according lo the formula. 



^ K (A - - x) a (B - x) b 



a and b being exponents, the values of which were 

 determined from Ihe experiments. The toxicity at the 

 time 0, viz. that of pure tetanolysin was put like 84.3, 

 0.3 cc. of this solution being necessary to produce the 

 given htemolysis (7), when the experiments had been 

 kept at 37 C during 1 hour (2,5 / blood). The toxicity 

 of all the solutions used for the experiments has been 

 determined in this way. In the next tables the toxicity 

 (A - x) has been calculated from the supposed equili- 

 brium (I) taken as zero, viz: if this (I) for instance corre- 

 sponds to the toxicity 1, then A x == 7 signifies, that 

 the toxicity, really observed was 8, that is that 1. 43 cc. 

 of the solution in question was necessary to produce 

 the given haemolysis. Still this mode of calculation is 

 not applied lo the ciphers observed in the case of the 

 longest time of reaclion, which were exclusively used 

 for the determination of I. 



The calculated figures are found under the suppo- 

 sition a - - 2, and b == 0, viz. that the differentialequation 

 for the velocity of reaction had this form: 

 d\ 



W i A i- \2 



l\ (A X ;* 



dt 



or integrated: 



1_ _ K a - n 



.r \ * 1 / 



