VI 



immunised against diphtheria toxin, and Dean') got 

 similar results. The third research on these lines was 

 made by Morgenroth 2 ), who showed that the rcnnin 

 immunisation followed an nndnlatory course like that 

 of diphtheria and tetanus. Later on Bulloch 3 ) demon- 

 strated, that in the case of the haemolvsins similar 



*> 



phenomena occurred. The last antibody drawn under 

 this kind of experiments is that of botulismus, studied 

 by Forssman and Lundstrem 4 ), who found the anti- 

 botulismus curve very like that of diphtheria and teta- 

 nus antitoxin. 



The difficulties in procuring an extensive and reliable 

 material are mainly caused by the necessity of deter- 

 mining the antibodies by means of animal experimen- 

 tation. For our investigations we therefore searched for 

 such antibodies, the testing of which could readily be 

 performed in test tubes and found the agglutinins very 

 suitable for this purpose. 



The following experiments are made with typhoid 

 and cholera agglntinin and can be classified into three 

 groups. In the first is treated the production of agglu- 

 tinin during various kinds of active immunisation; in 

 the second the fate of agglutinins, introduced in the 

 organism (passive immunisation). At last we have ex- 

 amined how an animal passively immunised reacts 

 during attempts on active immunisation. 



!) George Dean: Problems of diphtheria immunity. Transact of the Pathol. Society 

 of London. 1900. 



2 ) J. Morgenroth: Ueber den Antikorper des Labcnzyms. Centralhlatt f. Bad. elc. 

 XXVI. 1899. 



3 ) William Bulloch: On the nature of ha-molysis and its relation to bacteriolysis. 

 Transact, of the Pathol. Society of London. 1901. 



4) Forssman et Lundstr0m: Sur la marche de la courbe d'antitoxine dans 1'immu- 

 nisation active centre le botulisme. Annalcs de 1'Institut Pasteur 1902. 



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