VI 



inimunisedwith 24h 48h cultures in small and increasing 

 doses; the former intraperjtonealty, the latter subcuta- 

 neously. During the experiments small amounts of 

 blood were withdrawn from the ear vein of the rabbits 

 and the jugular vein of the goats. 



Of such experiments a great number was made which 

 gave very uniform results. Examples are indicated on 

 the curves 1 4. 



*, ,, ..., , Curve No. 2a re- 



presents a goat 

 which during se- 

 veral years had 

 been immunised 

 with B. typhos cul- 

 tures. On the Y 5 

 1900, when the ag- 

 glutinating power 

 of the serum was 

 33, 100 cc 24h B. 

 typhos. culture 

 were injected. The 

 next day showed 

 a decrease to 22, 

 but on the follo- 

 wing days an in- 

 crease first slow 

 and then rapid 

 was observed, un- 



Curve No. 2b 



til the maximum 

 was reached the 



9th day after the injection; thereafter a continual fall 

 occurred. 



For the next experiment (curve 3) served a goat, im- 

 munised with Vb. cholerae cultures, whose agglutinating 



- 11 - 



