VI 



nothing to say in this respect. They were undertaken 

 at the animals mentioned on curve No. 10. The rab- 

 bits T and 8, which had reached exactly the same agglu- 

 tinating power after injection of respectively rabbit 

 and goat serum, got both on the 31 / G 5 cc. of a 24h ciil- 

 lure of B. typhos. It is evident, that the agglutinating 

 curves were not in the least influenced, but continued 

 in quite the same way as those of the two other rab- 

 bits and ?, on which no culture had been injected. 



Quite corresponding results were obtained from the ex- 

 periments on goats (curve No. lOb). Having received the 

 same quantity of rabbit and goat serum the goats I and II 

 showed the same agglutinating power (8). As it appears 

 from the curve the injection of 40 cc. of a 24h culture of 

 typhoid fever bacilli produced not the slightest deviation 

 of the curve. 



Amongst the other experiments only the following 

 need to be quoted. On the 30 / 5 rabbit B, curve No. 

 8, had fallen down to 12. On this date 5 cc. 24h B. 

 typhos. culture were injected and produced no reaction 

 at all: the curve continued its fall without interruption. 

 To make sure, that this was not due to a special indi- 

 vidual absence of agglutin producing faculty in the rab- 

 bit in question, we waited until its serum for about 2 

 weeks had given no agglutinating reaction at all, and 

 then a new injection of quite the same dose of culture 

 was given. This time was observed a well marked reaction 

 of the same character as that, which was usually found in 

 the case of fresh animals. 



Another experiment quite similar to this was under- 

 taken on a goat (curve No. 7). 15 days after an injection 

 of typhoid agglutinin from goat the agglutinating power 

 was reduced to 15; at this time 100 cc. of culture pro- 

 duced no change at all. Some months later on the con- 



2'J 



