VI 



menls have been performed with the object of deciding 

 on which point the agglutinin acquires the properly to 

 prevent the microbes from producing the typical curve. 



The coagulation is the most conspicuous phenome- 

 non, which takes place in the agglutinin holding blood 

 after its removal from the organism in which it is pro- 

 duced. To clear up the significance of this phenomenon 

 it was necessary to examine the action of the same ag- 

 glutinin before and after coagulation. 5 goats were used 

 for the first series of experiments of this kind. 



Goat No I got on the 8 / 5 40 cc. B. typhos. culture. 

 The 17 / 5 when its serum was supposed to have reached 

 the maximum of agglutinin the following experiments 

 were carried out: 



a) From the jugular vein was taken with a syringe 

 100 cc. of blood, which immediately, and before 

 any coagulation took place, were introduced into 

 the jugular vein of goat II. 



b) From the same animal 200 cc. of blood were with- 

 drawn and after defibrination injected intravenous- 

 ly into goat III. 



c) 40 cc. infusion of leeches were injected in the jugu- 

 lar vein of No. I. 1 / z hour later 100 cc. of blood 

 were taken, mixed with 4 cc. infusion of leeches 

 and centrifugalised. The completely clear and fluid 

 plasma was thereafter introduced into the circu- 

 lation of goat No. IV. 



On the next day the animals were getting on all right. 

 Goat II, III, IV and a fresh goat V, which served as con- 

 trol, got an injection of 20 cc. 24h culture of B. typhos. 

 Only a trace of oedema on the place of injection was 

 observed, but no other morbid phenomena. In this re- 

 spect no difference between the animals could be disco- 

 vered. 



33 - 



