VI 



As it appears from curve 12 a typical agglutinin curve 

 took place in all the goats II, III, IV and also in the 

 control animal goat V. (see curve 5). The curve was 

 certainly very different in these four animals, but with 

 our present knowledge to the great individual differen- 

 ces no importance can be placed upon this fact. 



This experiment is unsuccessful so far as the animal, 

 which was passively immunised with defibrinated blood 

 also gave a curve, and therefore no conclusion could be 

 drawn from the other experiments with transfusion of 

 blood and plasma. This phenomenon differs quite from 

 all our earlier observations, and the explanation is pro- 

 bably, that there was a too small augmentation in ag- 

 glutinating power produced in the goats II, III and IV 

 by the injections of agglutinin. 



It appears from the curve, that goat No. II after the 

 injection showed 17. The serum of goat No. Ill, which 

 before the injection possessed a normal agglutinating 

 power of 28,5, increased thereafter to 50, and No. IV 

 reached 29. 



This experiment being unsatisfactory, a second was 

 carried out with a considerably greater quantity of ag- 

 glutinin. This was procured from the actively immuni- 

 sed goats I, II and III, which on the 12 / 6 each got a sub- 

 cutaneous injection of 100 cc. 24h B. typhos. culture. 



9 days later on the 21 /o following experiments were 

 made. 



a) From the jugular vein of goat I 350 cc. blood were 

 transferred directly into the jugular vein of a quite 

 fresh goat No. VI. No coagulation at all took place. 



b) From the jugular vein of goat II 400 cc. of blood 

 were drawn and defibrinated. After removal of 

 the fibrin by filtration through gauze, the blood 

 was set on a cool place till the following day 22 /e> 



35 



