VI 



and then 280 cc. thereof injected intravenously 

 upon another fresh goat No. VII. 

 c) From the jugular vein of No. III. 400 cc. of blood 

 were drawn and immediately after injected into the 

 same animal (auto-transfusion). To prevent coagu- 

 lation in the canula and the syringe, these were 

 previously filled with 4 cc. of a very weak infu- 

 sion of leeches. 



On the 8a /o 100 cc. 48h B. lyphos. culture were injec- 

 ted on goat No. VI, 24 hours after the transfusion. 



On the same day goat VII and III got similar amounts 

 of culture ca 10 hours after the injections. 



The result of the experiment was, that goat VI and 

 VII after injection of resp. uncoagulated and defibrinated 

 blood gained a comparatively high agglutinating power. 

 This was resp. 167 and 567 at the moment, when the 

 culture was introduced. No agglutinating curve look 

 place, (see curve No. 12). 



A typical one on the contrary occurred in the case 

 of goat No III, on which an auto-transfusion was under- 

 taken. After another auto-transfusion a quite similar 

 result was obtained. 



On curve No. 12 a corresponding experiment with a 

 rabbit is given. 



The 23 / 6 , 9 days after an injection of 5 cc. B. typhos. 

 culture, 20 cc. of blood was drawn from the jugular vein 

 of a rabbit; as this animal previously had got an injec- 

 tion of 10 cc. of infusion of leeches no coagulation took 

 place. 10 cc. of plasma were easily obtained through 

 centrifugalising and injected intravenously into another 

 rabbit Q. The agglutinating power of the serum was 

 thereby increased to 143. On the following day, when 

 it had fallen down to 100, 5 cc. of a 24h B. typhos. cul- 

 ture was injected and produced no agglulinin curve. 



36 



