VI 



sed animals gradually to examine if a culture would 

 produce an agglutinin curve, when such animals pre- 

 viously had got an injection of 1) their own serum, or 

 2) their own plasma, or 3) their own blood (auto-trans- 

 fusion). As regards the first two points these problems 

 can only be solved, when the serum, resp. plasma is 

 kept for a comparatively long time. Before the agglu- 

 tinating power of the animal, experimented on, has de- 

 creased to a considerable degree, the serum or plasma 

 injected would in no cases be able to increase the 

 amount of agglutinin to a sufficient extent. 



It is a matter of course, that such an increase can- 

 not be obtained in the case of an auto-transfusion, and 

 therefore the experiments of this kind have all given 

 "agglutinin curve". The examination of the two first 

 questions is not yet finished. 



The above problems can scarcely be solved by exa- 

 mination of the agglutinating power of the blood 

 alone, the peculiar action of typhoid and cholera serum, 

 which has been the object to these last experiments, 

 probably being due to other antibodies of these sera. 



Conclusions. 



1) The find nations in the agglutinating power of 

 the blood of an actively immunised animal, pro- 

 duced by a single injection of a culture of B. ty- 

 phos. resp. Vb. cholera 1 , can be expressed by a 

 regular curve. In this 3 phases can be distin- 

 guished : 



1 phase. 3 6 days almost imperceptible de- 

 crease of the agglutinating power or no change. 



2 phase. 3 6 days. An increase with a max- 



38 



