VII 



and diphtheria. It was herafter very likely, that a simi- 

 lar rule would apply to the other members of the said 

 group of microbes, and I have therefore, summoned by 

 Dr. Th. Madsen, undertaken a series of experiments in 

 that line. 



In this paper only a brief statement of some of my 

 results will be given; more detailed account for the 

 experiments shall later be published. 



The chief object for these researches was an inve- 

 stigation of the phenomena, which occurs into organims, 

 actively immunised against Bad. coli. I have also tried 

 to examine the influence of different factors upon the 

 course of immunisation, viz. the virulence of the employed 

 culture, and the results of varying modes of immuni- 

 sation. 



The way of proceeding was quite similar that, used 

 of Jergensen and Madsen in their experiments with 

 typhoid and cholera agglutinin. Different cultures of 

 B. coli were used, both for the immunisation and for 

 the determination of the agglutinin. A 24h 48h fresh 

 or boiled culture was injected intraperitoneally on rab- 

 bits; this species served for all the experiments. The 

 bloodsamples were drawn from an ear vessel, vein or 

 arteria, and the measurement of the agglutinin was per- 

 formed by the test tube method, whereafter the re- 

 sults in arbitrary units was indicated in the form of 

 curves. 



During the experiments a question of certain impor- 

 tance was examined, viz. the differences between dead 

 and living coli cultures, when acted upon by the same 

 amount of agglutinin. For this reason most of the 

 curves are made by means of both kinds of culture, 

 and thus a good material was procured to decide the 

 said question. 



