VII 



Exp. I. 



For the purpose of examining the influence of one 

 single injection, 6 cc. of a boiled culture was on the 

 16 / a introduced into the peritoneum of a rabbit. Of 

 this culture 0,5 cc. killed rabbits in 8 10 hours, but 

 after the boiling the culture seemed to have lost nearly 

 all toxicity, the rabbit to be examined showing no mor- 

 bid phenomena at all. 



The agglutinating power of the serum, which at the 

 beginning of the experiment was 0, increased after the 

 injection first slowly, thereafter more rapidly and reached 

 the maximum 17 days after the injection, whereafter a 

 gradual decrease occurred. 



On curve 1 

 is represented 

 the fluctua- 

 tions in the 

 agglutinating 

 power of this 

 rabbit, measu- 

 red both with 



living and dead cultures. It appears, that the values 

 obtained by means of the latter, are somewhat lower. 



Exp. II. 



In this experiment a rabbit was regularly immunised 

 with small doses of dead culture. During a period from 

 the 1(i / 2 to the l / 3 2 cc. of boiled culture was injected 

 every second day. Under this treatment an augmenta- 

 tion in the agglutinating power was observed, this being 

 before the injection took place. During these the in- 

 crease was comparatively small, but thereafter a con- 

 siderable rise occurred with a maximum 10,000 on the 

 13th day after the last injection. (Curve No. 2). 



Curve No. 1. 



