VII 



boiled cultures and later on living cultures were em- 

 ployed. The microbe used was the same as in experi- 

 ment I and II. At last 5 cc. of 24h culture were injected, 

 0,5 cc. of which killed control animals in 810 hours. 

 The leading features of the following agglutinin curve 

 (No. 4) were quite the same as the above mentioned. It is 

 still to be remarked, that the production of agglutinin in 

 this case was quite extraordinary; the maximum being at 

 333.333. 



In the curve 1,2 and 4 the agglutinin is determined both 

 with living and dead culture, and a considerable diffe- 

 rence may be noted. As a rule the values obtained with 

 dead cultures are only 25 50 % of the values obtained 

 by the living cultures; this indicates, that by the hea- 

 ting a great deal of the agglutinable matter in the bac- 

 teria is destroyed. 



It appears from communicated experiments, that the 

 production of agglutinin in rabbits, actively immunised 

 against bacterium coli, follows quite similar laws as 

 those observed in the case of Bacillus lyphosus and 

 Vibrio cholerae. 



8 



