X 



increased so much that they could be felt. Then the 

 weight decreased till death, or till the wound healed. 



The investigations which I am now going to describe, 

 have been made on in all thirteen guinea-pigs, and fol- 

 low the changes which took place from 3'/ 2 hours after 

 the inoculation up to the 35th day. 



The three first guinea-pigs weighed each 500 grammes, 

 the ten last ones 300 grammes. For inoculating I used 

 a 5 7 days old culture of Sacch. neoformans Sanf. on 

 a malt agar surface, suspended in a 0, 7 % solution of 

 common salt, in such a quantity that the liquid became 

 of a milky appearance. Of this milky fluid V 2 ccm. 

 was inoculated under the skin in the centre of the right 

 side of the abdominal walls. 



The animals were killed by chloroform at the end 

 of the stated periods, and the whole abdomen was pla- 

 ced, during 24 hours, in a saturated watery sublimate 

 solution; it was then, during 24 hours, washed in run- 

 ning water and was cut into horizontal strips, that were 

 hardened in alcohol of increasing strength and then 

 embedded partly in paraffin, partly in celloidin. The 

 paraffin sections were smoothed out with hot water 

 and mounted on slides by means of albuminous water. 

 On examining the sections it was necessary to use 

 many different staining methods. 



A combination of staining with lithium carmine and 

 Claudius' methyl violet picric acid [I] proved to be the 

 best method for showing the way in which the yeast 

 cells were embedded in the tissues. The receipt is as 

 follows: lithium carmine 2 min. - - hydrochloric alcohol 

 10-20 min. 1 % watery solution of methylviolet 



[GB] y 2 -l min. half-saturated watery picric acid '/ 2 

 min. quick rinsing with absolute alcohol - clove 

 oil for decoloration --xylol balsam. Between the diffe- 



