X 



rent stages of staining the preparation is pressed with 

 filtration paper. The results of this staining are very 

 fine, hut it can he used only with mounted paraffin 

 sections, the celloidin being unable to withstand the 

 clove oil treatment. 



Moreover, the following stains were used: W dyer is 

 fibrin staining [II] and elastin staining |III|; thionin, which 

 especially when using 1 % acetic acid between the stain- 

 ing and the dehydration with alcohol produces a very 

 fine preparation, hcematoxyline and van Gieson's stain 

 [V] or eosin [VI]; by way of experiment, as stated below. 

 Ehrlich's triacid solution [VII] and Ehrlich-Biondi-Hciden- 

 hain's slain mixture [VIIIJ. 



The normal structure of the abdominal walls of the 

 guinea-pig is principally as follows: the skin is hardly 

 1 mm. thick. It consists, on the outside, of several layers 

 of epithelial cells the first containing the pigment. The 

 connective tissue of the leather-skin is tight, with rela- 

 tively few elastic threads and a number of small vessels. 

 Under the skin there is a thin layer of loose connective 

 tissue with only few elastic threads, sometimes with a 

 number of fat cells. This loose layer of connective 

 tissue passes, without any definite division, into a fas- 

 cia layer of about Yio mm - m thickness containing a 

 number of elastic threads passing especially from one 

 side to another. The yeast cells were generally found 

 after the inoculation on the surface and in the upper 

 parts of this layer. Underneath there are four layers 

 of muscles, separated by thin layers of connective tissue, 

 the thickest between the first and second layers along 

 with a number of elastic threads mainly running length- 

 wise. The peritoneum is y 50 mm. thick and its inmost 

 layer is especially rich in elastic threads. 



As already stated, the greater part of the inoculated 



