X 



lar. Some of them have a two-lohed nucleus and are filled 

 with granules, which are stained yellow red by van 

 Gieson's method and clearly light red by eosin. 



Whereas the absorption of the yeast by these cells 

 is quite an exception, a great many injected Saeeharo- 

 myces are seen to have been ingested by the polynu- 

 clear cells which apparently, at least, are without granules. 

 Often 5 6, sometimes 8 10 even 14 yeast cells are 

 found in one white corpuscle. Whilst the leucocytes at 

 their invasion into the tissue are, on an average, 8 ;J . 

 in diameter they will, under the phagocytose, swell 

 and reach 10 14 ^ 



The cells of the connective tissue are of a normal 

 appearance. On this stage the yeast cells already com- 

 port themselves in a remarkable way when treated after 

 Weigert's fibrin staining method. While the contents 

 of a single yeast cell on cover-glass preparations from 

 culture are stained uniformly blue, the greater part of 

 the yeast cells which has remained that short time in 

 the animal tissue is discoloured; only a single, rather 

 large, round, sharply outlined, dark blue granule is 

 seen in the quite regular, round cell. At a weaker 

 discoloration the protoplasm still appears of a light 

 blue, and we may then see that this granule is no 

 nucleus, for in another part of the cell, immediately 

 under the membrane, we find the characteristic lenticu- 

 lar protuberance denoting the room of the nucleus (s. Fig. 5). 



This characteristic phenomenon is evidently not due 

 to the sublimate fixation, as it does not appear on co- 

 ver-glass preparations of yeast cells from cultures pre- 

 viously treated with sublimate. 



If stained by Claudius' method the yeast cells 

 are coloured uniformly blue; they appear regularly 

 round and are 4 6 . in size. 



