X 



Guinea-pig 6 ('& hours}: 



Whereas till now the yeast cells have shown no 

 signs of development, and most of them seem to have 

 decayed, they begin now, in guinea-pig 6, that is, forty- 

 eight hours after inoculation, to change in a charac- 

 teristic way. In the following I wil speak of these 

 transformed fungi as ,,tissue yeast cells" as distinct from 

 the inoculated ^culture yeast cells". 



It was often pointed out, first by Busse, later by 

 Sanfelice, Curtis, a. o., that the yeast cells in the tissues 

 looked quite different to what they did in culture, and 

 further stained in a different way. 



Well, this transformation thus begins in guinea-pigs 

 about forty-eight hours after inoculation. Among the 

 original 4 6 .j. sized yeast cells with thin walls stained 

 uniformly dark blue a few up to 20 y. sized cystoid, 

 granular, light blue stained cells are seen; in the 

 walls of these cells several layers may be distin- 

 guished, the outmost appearing as a thick homogeneous 

 capsule (s. Fig. (5). The first of these tissue yeast cells 

 are seen to be surrounded by the above-mentioned 

 circle of mononuclear wandering cells, and seem to be 

 free. In other places a few appear larger than the cul- 

 ture yeast cells. They stand out clearly on being stained 

 with thionin, which gives them a strong red violet tint. 

 They also lake a much deeper colour than the de- 

 caying culture yeast cells when stained with neutral red. 



It cannot with certainty be decided from the prepara- 

 tions at hand whether these commencing tissue yeast 

 cells were ingested by the colourless corpuscles without 

 succumbing or whether they entirely escaped the phago- 

 cytes. Either case might happen. In the former case 

 the yeast cells must necessarily have burst the colour- 

 less corspuscles as they are always free. 



11 



