X 



The results of these investigations may be resumed 

 as follows: 



After subcutaneous inoculation of Saccharomyces 

 neoformans Sanf. in guinea-pigs a lively emigration of 

 polynuclear colourless corpuscles begins after a few 

 hours. These corpuscles gather round the yeast cells 

 that are lying in a heap, and ingest them. As soon 

 as the greater part of the yeast fungi has been taken up 

 by the cells, the emigration stops having entirely ceased 

 eight hours after inoculation. 



Besides the non-granular, polynuclear, white cor- 

 puscles a few are seen with acidophil granules. Their 

 nucleus is, as a rule, two-lobed. They take a small 

 part only in the phagocytosis, disappearing very 

 soon. 



After the lapse of 18 24 hours a number of mononu- 

 clear, non-granular, wandering cells are seen in the 

 adjoining connective tissue without any connection with 

 blood-vessels. These wandering cells seem due to a 

 direct division of the connective tissue cells. They are 

 penetrating into the yeast heap among the polynuclear 

 phagocytes; like these they ingest yeast cells and, in a 

 few places, they seem to fuse into giant cells [s. Fig 3]. 

 The latter appear already after 3 4 days. 



Gradually fibroblasts make their appearance, due, as 

 it seems, to transformation of mononuclear wandering 

 cells. The fibroblasls will either form a ring round 

 the yeast cell heap or divide the same in smaller groups. 

 Some of the yeast cells now alter their appearance. 

 They increase in size, their staining alters, many of 

 them get capsules; from cull lire yeast cells" they be- 

 come ,,tissue yeast cells". These altered fungi will al- 

 ways lie free and isolated, spread in the tissue. Later 

 on, they increase greatly in number forming little heaps 



17 



