- 36 - OPALJNA. 



65. Living Animal. Make a fresh preparation of the contents of a toad's rectum in 

 G'(J5 per cent NaCl ; the large Ciliates present are Opalina. Draw an individual to show ; 

 ectoplasm ; endoplasm ; cuticle ; cilia. 



66. Stained Animal. Irrigate the above preparation with iodine solution and draw 

 a specimen showing the many nuclei Examine the demonstration specimen of stained 

 individuals. 



