10 CONTENTS. 



Modified potato cultures. Esmarch dishes. Potato 



tubes. Preparation of Roux tubes. Laboratory work. 



Examination of colonies, macroscopic and microscopic. 



Transplantation of colonies. Stab cultures. The plan of 



study. Laboratory work. 



CHAPTER VIII. 



The Non-Pathogenic Bacteria . . . . .193 



Bacillus prodigiosus. B. Indicus. B. ruber of Kiel. 



B. rubidus. B. violaceus. B. fluorescens putidus. 



B. phosphorescens. Orange sarcine. Yellow sarcine. B. 



subtilis. B. mesentericus vulgatus. B. megaterium. B. 



ramosus. Proteus vulgaris. Bacterium Zopfii. Spiril- 

 lum rubrum. B. acidi lactici. B. butyricus. B. cyano- 



genus. 



CHAPTER IX. 



Bouillon, Agar, Milk and Modified Media. The 



Incubator and Accessories 232: 



Advantages of bouillon, agar and milk. Preparation of 



bouillon. Preparation of agar. Sterilization of milk. 



Modified media: peptonless agar, glycerin agar; glucose, lactose, 



litmus and serum media. Laboratory work. 



The incubator. Thermo-regulators. Gas-pressure 



regulator. Micro-burners. Koch's safety lamp. Ther- 

 mometers. 



CHAPTER X. 



Relation of Bacteria to Disease. Methods of Infec- 

 tion and Examination ...... 253- 



Infectious diseases. Infection and intoxication. 



Rules of Koch. Generation. Attenuation. Bacterial, 



fungous and protozoal diseases. Unknown causes. 



Methods of infection. Cutaneous application. Subcu- 

 taneous injection. Animal holders. Intravenous injection. 



Intraperitoneal. Intrapleural. Anterior chamber of 



the eye. Lymphatics. Intracranial. Infection along 



respiratory tract. Alimentary tract. 



Observation of infected animals. Keeping of record. 



Food and drink. 



Post-mortem examination. Sterilizing case for instru- 

 ments. Preparation of the animal. Examination of the 



eub-cutis. Peritoneal and pleural cavities. Removal of 



