290 BACTERIOLOGY. 



The following' are not stained by Gram's method: 



B. anthracis symptomatici (p. 298). B. pneumonias. 



B. cholerae g-allinarum. B. rhinoscleromatis. 



B. choleras suis. B. typhosus. 



B. coli communis. M. gonorrheas. 



B. icteroides. Spirillum Obermeieri. 



B. influenzas. Vibrio cholerae Asiaticas. 



B. mallei. V. Deneke. 



B. oedematis maligni (p. 300). V. Finkler-Prior. 



B. pestis bubonicae. V. Metschnikovi. 



The following 1 synopsis of the staining methods for 

 streak preparations will be of service: 



Cover glass preparation. 



Dry in air. 



Once through flame. 



Simple stain: Gram's stain: 



Add drop of water. Anilin-water gentian violet (3 to 



Add dilute dye (#-^ min.). 5 min.; if hot, 1 to 3 min.). 



Wash in water. Wash in water. 



Examine in water. Iodine in potassium iodide (3 to 



Dry in air. 5 min.). 



Mount in balsam. Wash in water. 



Decolor in absolute alcohol. 



Wash and examine in water. 



Contrast color(dil.eosin,few sec.). 



Wash and examine in water. 



Dry in air. 



Mount in balsam. 



The above examinations of hanging-drop and stained 

 preparations serve the purpose of demonstrating the pres- 

 ence of the anthrax bacillus in the different organs and 

 tissues of the body. The form, size, etc., of the bacillus 

 found under these conditions should be compared with the 

 characteristics of the organism when grown in pure culture 

 on the different media. For this purpose make hanging - 

 drop examinations and permanent simple stains of the 



