314 



BACTERIOLOGY. 



is placed in a position so that only a narrow slit remains 



open. By means of a pi- 

 pette or other arrangement 

 25 c.c. of concentrated 

 potash solution (1:4), are 

 added as rapidly as possi- 

 ble. The top is then closed 

 completely and the rubber 

 band and clamps applied 

 as before. 



Laboratory work. Liquefy 

 four glucose agar tubes by heat- 

 ing in the water-bath; then allow 

 to solidify in an upright position. 

 When cool make deep stab cul- 

 tures of the 4 pathogenic anae- 

 robic organisms and then place 

 the tubes at 37. 



If the agar in the tube is 

 less than one inch deep, it will 



FIG. 52. 



FIG. 53- 



FIGS. 51-53. The author's apparatus for the culture of anaerobic bacteria. FIG. 51.. 

 Bottle for tube cultures. FIG. 52. Apparatus for Petri dishes or tubes. gas or pyrogal- 

 late method. FIG. 53. Apparatus for plates or tubes, gas, pyrogallate or vacuum method. 



