316 BACTERIOLOGY. 



a tubercle from a guinea-pig inoculated with tuberculosis should be 

 used. The material should be thoroughly spread over the surface of 

 the medium. 



2. Streak cultures of the aviary tubercle bacillus on inclined 

 glycerin agar and on glycerin potato as above. 



3. Streak culture of the Achorion Schonleinii (the fungus of 

 favus) on ordinary inclined agar. 



4 Streak culture of Actinomyces (the fungus of lumpy-jaw) on 

 ordinary inclined agar. 



5. Streak culture of the fungus of Madura-foot on inclined agar 

 or glycerin potato. 



After these inoculations have been made the cotton plug of each 

 tube is cut off close to the mouth of the tube. The end of the tube is 

 then rapidly turned in a flame till the cotton changes color. The tube 

 is then sealed either with a rubber cap (previously soaked in mercuric 

 chloride), or with sealing-wax, or with paraffin of a high melting 

 point, 56. Ordinary corks can be used to advantage provided they 

 are first immersed in mercuric chloride solution and steamed for at 

 least i hour. The heated, slightly charred cotton plug is pushed into 

 the tube by means of sterile forceps. The softened, sterile cork is 

 then inserted. The sealed tubes are then placed in the incubator at 

 39 for several weeks. 



Instead of sealing the tubercle cultures air-tight, it is advisable 

 to employ a cork through which passes a drawn-out capillary tube 

 {Fig. 54). 



The Staining of Flagella. 



The locomotive organs of bacteria, as described on p. 

 35, are extremely thin, wavy whips or flagella. These can- 

 not be seen in the unstained preparations, although under 

 favorable conditions they can be demonstrated by photo- 

 graphy. Only very rarely can motion be observed in the 

 liquid immediately surrounding the organism. In simple 

 stained preparations the flagella are likewise invisible, in- 

 asmuch as they do not readily take up the dye. The pres- 

 ence of whips or flagella can, therefore, be satisfactorily 

 demonstrated only by the aid of special staining methods. 

 The procedure as employed below is essentially that of 

 Loftier. 



