APPENDIX 



General Methods of Preserving and Hardening Tissues and Organs. 1 The 

 fluids which are most commonly used are alcohol, chromic acid solution 

 (1 in 500), picric acid solution (saturated), bichromate of potash solution 

 (2 per cent.), Miiller's fluid (bichromate of potash 2| parts; sulphate of soda 

 1 part; water 100 parts), and bichromate of ammonia (2 per cent.) The 

 following are the methods of hardening the several tissues and organs which 

 are found to give the best general results : 



Tissue or Organ 



Bladder .... 

 Blood-vessels . 

 Brain 



Elastic ligament 

 Embryos .... 

 Eye . . . . . 

 Eyelids .... 

 Ganglia .... 

 Heart .... 

 Injected organs 

 Intestine .... 

 Kidney .... 

 Lachrymal gland 

 Larynx .... 

 Liver .... 

 Lung . . . . 

 Mammary gland 

 Marrow of bone 

 Muscular tissue, striated . 



non-striated 



Nerve .... 

 (Esophagus 



Ovary .... 

 Pancreas .... 

 Retina .... 

 Salivary glands . 

 Sclerotic and cornea . 

 Skin . 



Hardening Fluid 



Chromic acid. 



Alcohol, or bichromate of potash. 



Bichromate of ammonia. 



Bichromate of potash. 



Chromic acid or picric acid. 



Miiller's fluid. 



Alcohol. 



Picric acid. 



Alcohol, or bichromate of potash. 



Alcohol. 



Distend with chromic acid. 



Bichromate of potash. 



Alcohol. 



Chromic acid. 



Bichromate of potash. 



Distend with chromic acid. 



Alcohol. 



Alcohol. 



Bichromate of potash. 



Chromic acid. 



Picric acid. 



Distend with chromic acid. 



Chromic acid. 



Alcohol. 



Miiller's fluid. 



Alcohol. 



Alcohol, or Miiller's fluid. 



Alcohol. 



1 Methods of preparation required for special purposes are given in the Lessons. 



K 



