I.] DISSECTION OF A RABBIT AND OF A DOG. 33 



the muscular surface downwards, stretching it 

 slightly, and place it in alcohol about 75 p. c. for 

 about an hour, then remove to 95 p.c. alcohol for 

 a fortnight ; keep in 75 p.c. alcohol. 



2. Cut out of the small intestine a piece about two 

 inches long ; tie into each end a short glass tube 

 with lumen about 5 m.m. in diameter, over the 

 free end of each glass tube slip a piece of india- 

 rubber tubing ; by means of a syringe wash out 

 the piece of intestine with NaCl *6 p. c. for 

 about twenty seconds, then inject chromic acid 

 *2 p. c. When the salt solution has been displaced 

 by chromic acid tie or clamp the peripheral piece 

 of tubing, inject a little more chromic acid to 

 distend slightly the intestine, and tie or clamp 

 the central piece of tubing. Place the distended 

 intestine in ten times its bulk of chromic acid 

 *2 p. c. In two to three days cut off both ends 

 of the intestine, cut it open longitudinally, and 

 place it in fresh chromic acid '2 p. c. ; in about 

 ten days place it in water for some hours, and 

 then in alcohol 30 p.c. ; on the next day transfer 

 it to alcohol 50 p.c. renewing the alcohol as long 

 as it becomes coloured and finally place the tissue 

 in strong spirit. 



3. Take a piece of costal cartilage about 5 m.m. 

 long and place it in about 10 c. c. of saturated 

 aqueous solution of picric acid ; after about ten 

 days wash it well with water and place for a 

 day in 50 p.c. alcohol, then transfer to strong 

 spirit. 



L. 8 



