IV.] HYALINE CARTILAGE. 53 



d. Along the cut edge some cavities from which 



the cells have fallen out may generally be 



seen. 

 c. Except at the free edge, the cells are two or 



more layers deep. 

 /. The matrix is hyaline or faintly granular, 



and is comparatively (cp. infra 4, 7) in 



small quantity. 



If the newts are not quite young the cartilage 

 will vary from the above in the quantity of ma- 

 trix, the shape and arrangement of the cells, and 

 in the cells possessing small fat globules and 

 often two nuclei. 



2. Irrigate the piece with acetic acid 1 p.c. 



The following changes will be seen as the irriga- 

 tion proceeds : 



a. The nucleus becomes much more granular 

 and distinct. 



b. Granules appear also in the cell-substance, 

 and more or less hide the nucleus. 



c. The cell-substance becomes transparent owing 

 to the disappearance of the granules. 



d. The cell-substance shrinks from the matrix, 

 and presents a granular-looking finely ser- 

 rated border. Note the space thus formed 

 between the cell and the matrix. 



3. Place a small piece in gold chloride solution *5 

 p.c. for about half an hour, (until it is of a light 

 yellow colour,) then wash well with water, and 

 place it in a vessel containing water just acidu- 



