V.J CONNECTIVE TISSUE. 59 



and tease this out as above; the tissue should 

 be kept moist with normal salt solution but 

 no more than is necessary should be added. 

 Place a small drop of salt solution on a cover 

 slip and let this fall gently on the tissue. 

 Observe the wavy bundles of fibrillaB; the 

 outlines of the fibrillse are not very distinct 

 but they may be seen to run parallel to one 

 another; owing to their small size and to 

 the cementing substance between them 

 special preparation is required to isolate the 

 individual fibrillae completely. 



b. Irrigate with acetic acid (1 to 5 p.c.). The 

 fibrilloe disappear, and the whole tissue swells 

 up into a transparent gelatinous mass, in 

 which hardly anything can be distinguished 

 except a few elastic fibres similar in general 

 characters to those of A. 1, but much finer. 



c. Pull up with fine forceps the thin connective 

 tissue lying between the muscles of the thigh 

 of a rabbit or frog, cut off with scissors a 

 small piece and tearing it as little as possible 

 spread it out on a slide with the aid of needles, 

 breathing on it if necessary to prevent it from 

 drying. Mount as in a or press a dry cover- 

 slip gently on the film and allow saline 

 solution to run under the cover-slip; thus 

 the film is kept extended. 



It is chiefly composed of wavy bundles of fibril- 

 Ia3 similar to those seen in (6) ; the bundles 

 are of various sizes and run across one another 



