64 ELEMENTARY PHYSIOLOGY. [v. 



which, in some cases, are seen to be connected 

 with the processes of other similar cells, (cp. 

 B. 1, 2.) 



c. Larger, more rectangular cells, generally 

 without evident processes, with oval nuclei, 

 lying over the bundles of fibrous tissue often 

 in rows or patches; on a side view these 

 appear as thin long cells. 

 This preparation should be preserved for the 

 observation of fat tissue (D. 5). 



6. Snip off a small piece of the jelly-like subcuta- 

 neous tissue in a foetal or new-born mammal 1 , 

 and mount as in A. 2, c. 



Note the pale granular cells of various shapes, 

 some not unlike those seen in B. 1. To make 

 the cells and their nuclei more distinct stain 

 with Spiller's purple in the manner given in A. 

 2 (d), or irrigate with dilute acetic acid watch- 

 ing closely the early changes produced. 

 This may also serve to shew the development of 

 fat-cells (D. 6). 



7. Note in the section of costal cartilage prepared 

 in Lesson IV. 6 the connective tissue layer out- 

 side and closely attached to it forming the peri- 

 chondrium ; in places sections of tendons running 

 into the cartilage will probably be seen. 



1 An animal just killed serves best, but one preserved in picric acid 

 or Muller's fluid will answer the purpose ; if it is desired to observe 

 the forming fat-cells the animal must not have been transferred to 

 alcohol stronger than 50 %. 



