G6 ELEMENTARY PHYSIOLOGY. [V. 



3. Take a small piece of omentum, which has been 

 kept for some time in alcohol, and place it in 

 haematoxylin until it has become well stained. 

 Wash it with spirit, place it on a glass slide, and 

 tease it 'out if it is too thick. Remove as much 

 of the spirit as possible with blotting-paper. 

 Cover the tissue with a mixture of creosote 

 (1 part) and turpentine (4 parts), and let it 

 remain until it is completely transparent, chang- 

 ing the fluid and gently warming if necessary. 

 Remove the excess of turpentine, cover the tissue 

 with a drop of Canada balsam, and put on a 

 cover-slip. 



Observe the groups of cells from which the fat 

 has been thus removed. Note the shrunken 

 outlines of the cells, the marked membrane, and 

 the presence in each cell of a deeply stained 

 nucleus. A small amount of protoplasm, also 

 stained, may be seen as in 4. 



4. In the gold chloride preparation of sub-cutaneous 

 tissue made above (B. 5), observe 



The network of capillaries in a small collection 



of fat-cells. 



The large, flat, connective-tissue corpuscles (B. 



5, c) in greater quantities near the groups. 



When the fat-cell group is spindle-shaped, these 



may often be seen proceeding in rows from the 



ends. 



In some fat-cells a nucleus and a small amount 



of protoplasm surrounding the fat may be seen. 



Do not mistake a deposit of crystals in the fat 



for a nucleus. 



