VI.] MODIFICATION OF CONNECTIVE TISSUE, ETC. CD 



to the surface of the cartilage, at the same time 

 bevelling the edges. In cutting the sections, 

 cover the tissue and the blade of the razor with 

 spirit (for convenience from a wash-bottle) and 

 remove the sections by means of a camel-hair 

 brush from the razor to a watch-glass. With 

 the platinum "lifter" transfer them to hsema- 

 toxylin and treat as in 7, Lesson IV. 

 It is to be remembered that if the chromic acid 1 

 has not been completely removed from the tissue 

 the staining will be very imperfect ; in this case 

 the sections should be placed in a little Na 2 CO 3 

 1 p.c. for a few minutes and then washed with 

 water before staining. Observe in the sections 



a. The softened bone passing into 



b. The thin layer of hyaline cartilage ; outside 

 this 



c. The arrangement of the cartilage cells in 

 rows, and the concomitant appearance of 

 fibres in the matrix, this gradually but 

 rapidly merging into 



d. Fibro-cartilage consisting of bundles of white 

 fibrous tissue and of rather large cells arranged 

 in rows between the fibrous bands; these cells 

 are like the cells of hyaline cartilage and 

 each has a thin hyaline capsule around it. 



e. Towards the outer part of the cartilage, the 

 capsules and cells become smaller and more 



1 Miiller's fluid, potassium bichromate and ammonium bichromate, 

 must similarly be completely removed from tissues which are to be 

 stained. 



