102 ELEMENTARY PHYSIOLOGY. [iX. 



frequently shaking. After 3 or 4 hours most of 

 the myosin of the muscle will have been con- 

 verted into syntonin or acid-albumin, and dis- 

 solved. Syntonin may be prepared in a similar 

 way from white of egg or from serum. 

 Filter and carefully neutralize the nitrate with a 

 weak solution of sodium carbonate (1 c.c. of HC1 

 1 p.c. requires about 1*5 c.c. of Na 2 C0 3 '1 p.c. to 

 neutralize it), a copious bulky precipitate of 

 syntonin takes place ; if too much alkaline salt 

 is added the syntonin will be converted into 

 alkali albuminate and redissolved (cp. 16). 

 Filter and wash the precipitate once on the filter 

 with water, then break through the filter paper 

 and wash the precipitate into a beaker with 

 water. The syntonin is thus obtained suspended 

 in water. Place 2 c.c. in each of 2 test-tubes. 



a. Add a little HC1 '1 p.c., the precipitate at 

 once dissolves and is not precipitated on 

 boiling; cool under a tap, and test for pro- 

 teids e.g. with acetic acid and potassium 

 ferrocyanide (cp. Less. ill. 16). 



b. Boil, cool under a tap, and add a little HC1 

 1 p.c.; the precipitate suspended in the water 

 has been coagulated by boiling and is no 

 longer soluble in dilute acids. 



16. Alkali Albuminate. 



Treat a little washed muscle as in 15 but with 

 NaHO 1 p.c. instead of with HCL On neutral- 

 izing a precipitate of alkali albuminate will be 



