188 ELEMENTARY PHYSIOLOGY. [XVII. 



C. PANCREATIC JUICE. 



Artificial Pancreatic Juice. 



1. Mince finely a pancreas from a just killed animal, 



pound it well with clean sand and add about 

 100 vols. Na 2 C0 3 '2 p.c. and a little thymol. 

 Place it in the warm for some hours to a day, 

 strain through muslin, filter through linen and 

 then through filter paper. 



2. Leave a pancreas moistened with water for a 

 day, then mince it well and add 10 vols. of 

 glycerine. When required for use add to a 

 small portion of this glycerine extract 10 to 

 20 vols. of Na 2 C0 3 1*5 p.c., shake, strain through 

 muslin and filter. 



3. Mince a pancreas and pound it with sand, for each 

 gramme of gland-substance add 1 c.c. of acetic 

 acid 1 p.c. and mix thoroughly in the mortar for 

 ten minutes ; add ten times its bulk of glycerine. 

 In a day or two a little strong solution of sodic 

 carbonate should be added to make the fluid 

 slightly alkaline. When required for use add 

 sodium carbonate as in 2. 



Properties. 



4. To about 5 c.c. of distilled water in a test-tube 

 add a drop of oleic acid and shake, the fatty 

 globules soon rise to the surface; add 5 c.c. 

 Na 2 CO 8 1 p.c.; a white precipitate of soap 

 forms; shake, the precipitate partially dissolves 

 and more completely or wholly on boiling. 



