XVII.] INTESTINE. BILE. PANCREATIC JUICE. 189 



Examine a drop of the fluid under the micro- 

 scope, no fat globules will be seen. 



5. Place in a warm bath two test-tubes, each con- 

 taining 5 c.c. Na 8 CO 3 1 p.c. ; melt a little fresh 

 butter in a porcelain dish over a flame and with 

 a warm pipette add an equal quantity (about 

 2'5 c.c.) of the melted butter to the fluid in each 

 test-tube. To one of these (a) add a couple of 

 drops of oleic acid. Shake the test-tubes and 

 replace them in the warm bath, examining 

 them from time to time; the fine emulsion 

 formed on shaking the fluids will last much 

 longer in (a) than in (6). The emulsion in this 

 case is much greater than with bile (cp. B, 6). 



G. With either of the extracts of 1 or 2, make 

 observations on the amylolytic ferment of the 

 pancreas similar to those made in Lesson xv. 

 8 13 on the amylolytic ferment of saliva. 



7. Test the proteolytic action of either of the 

 extracts 2 or 3 in a similar manner to that 

 in which the action of artificial gastric juice was 

 tested (Lesson xvi. C, 2) substituting 1 p.c. 

 Na 2 C0 3 for '2 p.c. HC1. 



In test-tubes A and D the fibrin will be unalter- 

 ed, in G it will be very slowly dissolved, in B it 

 will be rapidly dissolved, hence sodium carbonate 

 alone does not digest fibrin (A), trypsin alone 

 digests it very slowly ((7), trypsin in the presence 

 of sodium carbonate dissolves it rapidly (B), the 

 digestive power of trypsin being destroyed by 

 boiling (D). 



