APPENDIX. 379 



and set aside until the cloudiness which forms on 

 mixing the fluids disappears. 



Sections may be transferred to this from strong spirit, 

 they may however have to be gently warmed to 

 render them clear throughout. Before mounting in 

 Canada balsam, the sections should be examined 

 under a low power in creosote and turpentine to see 

 that the clearing process is complete; if they are 

 not, fresh creosote and turpentine should be added 

 and the sections warmed in it. Cp. also p. 75, foot- 

 note. 



Before mounting the sections in Canada balsam as 

 much of the clearing fluid as possible should be 

 removed by means of a piece of blotting-paper with 

 a clean cut edge. 



Canada Balsam. 



Put some Canada balsam into a capsule, and place it in 

 the warm chamber at about 65 C. for twenty-four 

 hours to drive off all water. Let it cool and dissolve 

 it in a sufficient quantity of chloroform or benzole to 

 make a fairly fluid solution; it should be kept in 

 a bottle with a ground glass cap fitting over the 

 neck of the bottle, instead of a stopper, as the 

 stopper is apt to become fixed in the bottle ; if any 

 balsam is allowed to get on the neck of the bottle 

 wet it with spirit and rub it off with a cloth. 

 Dammar may be used instead of Canada balsam. 



Canada balsam renders tissues much more transparent 

 than glycerine. 



Tissues stained in carmine or any watery solution 

 should be placed in alcohol of increasing strengths 



