BACTERIAL CELLULAR SUBSTANCE 



47 



mortem examination all animals dying from intraperitoneal 

 injection showed marked congestion of the mesentery, 

 omentum, peritoneum, and adrenals; also numerous ecchy- 

 moses and hemorrhagic effusions in the serous coat of the 

 stomach, intestine, and peritoneum. The spleen, liver, 

 and kidneys were slightly enlarged and dark. In the 

 kidneys a dark line sharply divided the cortex from the 

 medulla. The heart was in diastole. When mixtures of 

 diphtheria antitoxin and suspensions of the cell substance 

 in salt solution were injected into guinea-pigs, death followed 

 as promptly as when the dead germ only was given. One 

 thousand immunity units failed to protect against 10 

 minimum fatal doses of the cell substance as shown by the 

 following: 



TABLE I 



In these experiments the minimum lethal dose of the 

 cellular substance was 1 to 33,000, and 1 c.c. of antitoxin 

 was equivalent to 235 immunity units. 



With another sample of cell substance and of antitoxin, 

 the following figures were obtained: 



Amount 

 No. Weight, injected. 



1 257 55.39 mg. 



2 280 0.05 c.c. 



TABLE II 



Antitoxin. 

 2.5000 c.c. 

 0.0025 c.c. 



Toxin Anti- Immunity 

 cor. toxin, cor. units. Result. 



2.5680 1000 + 

 0.056 0.0028 1 



245 52.80 mg. control 



In this series No. 2 received 10 minimum lethal doses of 

 a filtered bouillon culture of the same bacillus from which 



1 Antitoxin for 250 grams body weight. 



2 Antitoxin for actual body weight. 



