66 PROTEIN POISONS 



Nicolle and Alilaire 1 give the following table, showing the 

 percentage of water, nitrogen, substance soluble in acetone, 

 and phosphorus in the bacterial cells named : 



* o o . 2 . _gy 



5 o IS "So a2 oA 



g .- 1 . s g . s a . ^45 jw 



Organism. -3 ^ -g g *1| X | -S a * 



^ ^8-3 ^-0 13 ^ 



Carbohydrates. In no case have we been able to detect 

 cellulose in the bacterial cell substance. Wheeler made 

 special search for it in sarcina lutea. Twenty grams of 

 substance was autoclaved with 25 parts (500 c.c.) of 10 

 per cent, potassium hydroxide at 120, first for thirty 

 minutes and then for an hour. There remained a consider- 

 able residue which no longer gave the protein reactions, 

 but did respond to the carbohydrate test with a-naphthol, 

 although it did not reduce Fehling's solution even after 

 prolonged boiling with dilute hydrochloric acid. Cellulose 

 could not be detected by any of the tests employed. 

 Schweitzer's reagent failed to dissolve it, and it gave no 



1 Annales de 1'Institut Pasteur, 1909, xxiii, 547. 



