BACTERIAL CELLULAR SUBSTANCE 8? 



Hydrolysis of the Non-poisonous Portion of the Cellular 

 Protein of the Bacillus Tuberculosis. Five hundred grams 

 of this non-poisonous bacterial split product, known in this 

 laboratory as "residue" or haptophor, was hydrolyzed. 

 The humus was found to constitute 29 per cent, of the air- 

 dried, or 40 per cent, of the moisture-free substance, the 

 percentage of moisture being 27.5. 



The distribution of nitrogen was studied with the fol- 

 lowing results: 



Percentage of nitrogen in residue 4.59 



Percentage of nitrogen in moisture-free residue . . . ... 6.34 



Percentage of nitrogen in ash-free residue 5.54 



Percentage of nitrogen in ash- and moisture-free residue . . . .8.29 

 Amount of nitrogen in the hydrolyzed fluid .... 18.980 grams 

 Amount of nitrogen in the extract of humus .... 1 . 888 grams 

 Amount of nitrogen in the extracted humus .... 2.030 grams 



Total .... 22.898 grams 



Total amount of nitrogen in the 500 grams of residue . 22.990 grams 

 Total amount of nitrogen in the products of hydrolysis . 22 . 898 grams 



Loss during hydrolysis . 092 gram 



Amount of nitrogen in the hydrolyzed fluid .... 18.980 grams 

 Amount of nitrogen in the extract of humus .... 1 . 888 grams 



Amount of nitrogen in the fluid to be esterified ... 20 . 868 grams 



No glutamic acid could be obtained from the residue. 

 The result of the fractional distillation is shown as follows : 



Temperature of bath. Yield. 



Fraction I 40 to 60 21. 5 grams 



Fraction II ..... 60 to 80 5.0 grams 



Fraction III 80 to 100 8.0 grams 



Fraction IV 100 to 130 9.0 grams 



Fraction V 130 to 170 10.0 grams 



By the hydrolysis of 500 grams of tubercle residue the 

 following substances were obtained in the amounts and 

 percentages given: 



Per cent, of Per cent, of 



Substance. Amount. residue. dry residue. 



Humus .... 145.00 grams 29.00 40.00 



Alanin . . . . 4 . 00 grams . 80 1.10 



Leucin .... 6.40 grams 1.28 1.76 



Valin .... 2.30 grams 0.46 0.63 



Phenylalanin . . 1.70 grams 0.34 0.46 



