THE CLEAVAGE OF PROTEINS WITH ALKALI 111 



and showing both Millon and biuret tests, but not seriously 

 affecting animals in usual doses. 



The nitrogen in a number of the crude poisons has been 

 determined by Gidley in this laboratory as follows: 



PERCENTAGE OF NITROGEN IN PROTEIN POISONS. 



Per cent, of N. 



Source of poison. in crude poison. 



Colon bacillus . . . . . 13.49 



Typhoid bacillus 11.52 



Tubercle bacillus 11.00 



Pyocyaneus 10.50 



RuberofKiel 10.495 



Subtilis ... 8.12 



Megaterium 8.595 



Proteus vulgaris 10.17 



Yellow sarcine 6 . 145 



Egg albumen (Leach) 13.74 



Serum albumin 10.48 



Edestin 12 . 78 



Zein 10.69 



Witte peptone 11.14 



De Chapoteaut peptone . 12.735 



To study the distribution of the nitrogen, determinations 

 were made in both the colon and albumin poisons, of the 

 ammonia nitrogen, the mono-amino, and diamino nitrogen, 

 by the method already described under cleavage with 

 dilute mineral acids. The following are the results: 



DISTRIBUTION OF NITROGEN IN PROTEIN POISONS. 



It will be seen that the greater part of the nitrogen is to 

 be found in mono-amino combination. From the phospho- 

 tungstic filtrates, from both the albumin and colon poisons, 

 containing the mono-amino acids, crystalline bodies were 

 obtained. Judged by the strong Millon test, ty rosin was 



