114 PROTEIN POISONS 



Fixed Inorganic N ash P ash Ratio 



Ash. ash. ash. N. P. free. free. N:P. 



Cell substance . ... 8.61 ... 10.65 2.87 



Haptophor . . 33.25 .. 26.08 5.56 2.34 7.52 3.99 2.38 



Prep. A. . . . 26.76 20.36 15.66 6.76 3.61 8.02 4.28 1.87 



Prep. B. . . . 35.34 .. 30.74 4.87 1.50 7.03 2.16 3.25 



Prep. D. . . . 15.38 15.05 8.48 4.95 2.25 5.41 2.46 2.20 



Prep. G. . . . 6.99 .. 1.66 4.65 1.74 4.73 1.77 2.67 



Prep. G. pur. . 5.50 5.50 1.36 3.43 1.35 3.48 1.37 2.53 



Prep. H. . . . 35.91 14.00 27.67 5.98 2.68 8.27 3.71 2.23 



Prep. K 2 . . . 7.57 .. 2.08 5.50 1.79 5.62 1.83 3.07 



Prep. M. . . 11.71 11.71 3.74 3.16 2.47 3.28 2.70 1.28 



Prep. M 2 . . 8.30 .. 3.47 5.35 1.58 5.55 1.64 3.39 



Explanation of the Table. Ash, residue heating at low 

 redness. Fixed ash, residue after heating to full heat of 

 powerful burner. Inorganic ash, ash less calculated amount 

 of PO 4 . N, nitrogen by Ivjeldahl-Groening method. P, 

 phosphorus by the Neumann method. N and P ash free, 

 reckoned free from "inorganic ash." N:P, quotient of 

 column 4 divided by column 5. A, portion of haptophor 

 dissolved by acid alcohol. B, portion of haptophor not 

 dissolved by acid alcohol. D, substance precipitated by 

 acid alcohol from solution of B in aqueous alkali. G, 

 substance precipitated by acid alcohol from aqueous solu- 

 tion of haptophor. H, obtained by concentration of the 

 alcoholic filtrate from G. K, substance precipitated by 

 dilute acetic acid from aqueous solution of haptophor. K 2 , 

 same as K, except that strong acid was used. M, precipi- 

 tated by alcohol from filtrate from K. M 2 , precipitated 

 from filtrate from K 2 . 



Leach states: "As these preparations are all mixtures, 

 the absolute values are worth nothing taken singly, but 

 the comparative values, especially the ratio of N to P, as 

 given in the last column, are of interest. The determina- 

 tions were made for the sake of tracing the nucleo com- 

 pounds. There are many indications of nucleic acid, but 

 the amount of both nitrogen and phosphorus is much too 

 small. The ratio between them is, however, quite within 

 the range for nucleic acids from other sources, as may be 

 seen by comparison in the following table. Moreover, the 



