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PROTEIN POISONS 



decidedly alkaline solutions were employed. It was soon 

 noticed, however, that the results obtained in the three 

 cases were very different. Thus, whereas in the neutral 

 or faintly alkaline solution the injection of 60 mg. of the 

 powder invariably killed, in the case of a stronger alkaline 

 solution the same amount did not cause a fatal result, 

 although the animals were very ill. From this fact it 

 became evident that some change had taken place in the 

 poison on standing in alkaline solution. In order to study 

 this change more in detail, experiments were conducted 

 with solutions of different degrees of alkalinity, with the 

 results found in the following tables: 



TABLE III. RESULTS WITH SOLUTION or POISON BARELY NEUTRALIZED 

 WITH SODIUM BICARBONATE AND PLACED IN INCUBATOR 



TABLE IV. RESULTS WITH SOLUTION OF POISON RENDERED DECIDEDLY 

 ALKALINE WITH SODIUM BICARBONATE AND PLACED IN INCUBATOR 



No of Dose of 

 animal, poison. 



1 60 mg. 

 (barely 



neut.) 



2 60 mg. 

 decided- 

 ly alk. 



3 60 mg. 



4 80 mg. 



5 120 mg. 



6 160 mg. 



Solution 



kept in 



incubator. 



Fresh. 

 Fresh. 



2 hours 

 4 hours 



24 hours 



3 days 



Weight 

 of pig. 



350 gm. 

 370 gm. 



325 gm. 

 310 gm. 



280 gm. 



350 gm. 



Result of 

 injection. 



Very sick 

 for 2 hours 



Not very sick 



Sick 



Time of 



death after 



injection. 



30 minutes 

 Recovered 



Recovered 

 Recovered 

 More than 



5 hours. 

 7 hours. 



