THE SPLIT PRODUCTS OF TUBERCLE BACILLUS 187 



cultures to daylight for a year, but this failed. The same 

 investigator tried formalin, with a like result. Bartel 1 

 made a brie of tubercle bacilli and lymph glands, and 

 Schroder tried a like experiment with spleen pulp, but 

 neither of these preparations proved effective. Calmette 

 and his students have tried preparations obtained by the 

 action of iodine and its salts upon tubercle bacilli, but 

 without success. Similar preparations with chlorine have 

 been tried by Mossu and Goupil. 2 Noguchi 3 stated: "The 

 inoculation of guinea-pigs with tubercle bacilli, which have 

 been killed by soaps (sodium oleate), develops in these 

 animals a complete or partial resistance to subsequent 

 inoculation with a virulent culture of the same strains 

 of bacilli. In short, a condition of immunity to tuberculosis 

 can be induced in guinea-pigs by the injection of an emulsion 

 of tubercle bacilli in oleic soaps." Zeuner, 4 following the 

 work of Noguchi, has used an extract of tubercle bacilli in 

 solution of sodium oleate in the treatment of tuberculosis. 

 Broil 5 found that guinea-pigs treated with this preparation 

 survived only a few weeks. Marxner 6 tried it on goats, and 

 found no evidence of infection in two cases on postmortem, 

 but Lowenstein 7 states that this is explained by the fact 

 that the animals were sectioned too soon after inoculation, 

 and adds that he sectioned goats three years after this 

 treatment, followed by inoculation, and found tubercular 

 cavities, the size of a man's head, in the lungs. Deycke 

 and Much 8 found that, "One part of tubercle bacilli is 

 dissolved in two parts of a 25 per cent, solution of neurin 

 when kept at 52 for twenty-four hours. This forms a 

 perfectly clear syrup which becomes cloudy on cooling. 

 We attribute this phenomenon to the presence in the bacilli 

 of fatty bodies with high melting-points." Later it was 



1 Wien. klin. Wochenschrift, 1905. 



2 Compt. Rend, de 1'Acad., 1907. 



3 Centralbl. f. Bak., 1909, lii, 85. 



4 Zeitschf. f. Tuberkulose, xv. 



5 Berlin tierarztliche Wochenschft., No. 47. 



6 Zeitsch. f. Immunitatsforschung, x, xi, xii. 7 Loc. cit. 

 s Beitrage z. klinik d. Tuberkulose, xv, Heft 2. 



