THE ANTHRAX PROTEIN 197 



that into rats from 5 to 12 c.c.; in guinea-pigs from 4 to 15 

 c.c.; in rabbits from 10 to 20 c.c.; and in one dog, 25 c.c. 

 was injected subcutaneously. In proportion to body weight 

 the mice received by far the larger injections. The experi- 

 ments indicate that in the peritoneal exudate of guinea- 

 pigs inoculated with anthrax there is no appreciable amount 

 of soluble toxin. 



2. Many guinea-pigs were inoculated with anthrax, and 

 directly after death their livers and spleens were removed 

 under aseptic conditions and rubbed up in a sterilized 

 mortar with sterile sand to which a little physiological salt 

 solution had been added. After thorough rubbing the 

 mixture was diluted with physiological salt solution and 

 filtered through a Chamberland tube under four atmos- 

 pheres of pressure. The filtrate was injected subcutaneously, 

 intravenously, and intraperitoneally into mice, rats, guinea- 

 pigs, and rabbits, and in every case without effect. 



3. Conradi, finding the preparation of collodion sacs 

 difficult, substituted for them the vegetable membranes 

 from phragmites communis, first used by Podbelsky. 1 

 These sacs, after sterilization, were filled with bouillon 

 cultures of the anthrax bacillus, and after laparotomy 

 under ether were placed in the abdominal cavities of animals. 

 The animals used were guinea-pigs, rabbits, and dogs, all 

 of which remained well, notwithstanding the presence of 

 these tubes containing virulent cultures of the anthrax 

 bacillus in their abdominal cavities. These experiments 

 satisfied Conradi that the anthrax bacillus does not produce 

 any soluble toxin, and he next turned his attention to 

 determining whether or not this organism possesses any 

 intracellular poison. 



4. The anthrax exudates obtained as described in the first 

 series, in quantities of from 5 to 6 c.c., were placed in test- 

 tubes, 0.5 c.c. of toluol added to each, the tube closed with 

 a sterilized cork, thoroughly shaken, and then allowed to 



1 To one experienced in the preparation of collodion sacs this must be 

 regarded as a clumsy substitution. 



