PROTEIN SENSITIZATION OR ANAPHYLAXIS 257 



was made into the brain, sudden death resulted. It will 

 be seen from the above that passive anaphylaxis may be 

 demonstrated in the recipient not only by general reac- 

 tion, as anaphylactic shock, but by local reaction also. 

 Even before the work of either Gay and Southard, or that 

 of Nicolle, v. Pirquet and Shick 1 had, by a reversed method, 

 demonstrated passive anaphylaxis. To each of three rabbits 

 they administered 10 c.c. of horse serum; twenty-four hours 

 later, two received each 2 c.c. of rabbit-antihorse serum, 

 and 1 c.c. of rabbit serum, all subcutaneously in the ear. 

 In the first two, edema resulted, while in the third there 

 was no effect. By this reversed method anaphylactic 

 shock may be induced. Pick and Yamanouchi 2 injected 

 2 c.c. of ox-serum subcutaneously in young rabbits of about 

 700 grams weight, and 14 days later 5 c.c. of rabbit anti-ox 

 serum intravenously, causing anaphylactic shock and death. 



Weil-Halle and Lemain 3 have observed both local and 

 general symptoms of anaphylaxis in both guinea-pigs and 

 rabbits when simultaneously on one side rabbit-antihorse 

 serum and on the other normal horse serum, were injected. 

 A few hours after such injections, the tissue around the 

 point of injection of the antiserum becomes edematous, 

 infiltrates, and becomes necrotic. Either speedy death 

 follows, or the animal becomes cachectic and dies after 

 two or three weeks. 



Kraus, Doerr, and Sohma 4 found that the blood-serum 

 of rabbits sensitized to the proteins of the crystalline lens, 

 renders the recipients anaphylactic to the same proteins, 

 and that this sensitization is strictly specific, and Doerr and 

 Kraus 5 have made a similar showing in bacterial anaphyl- 

 axis. Furthermore, Richet 6 has made like demonstrations 

 with the serum of animals sensitized to mytilocongestion 

 and crepitin. 



1 Die Serumkrankheit, 1906. 



2 Zeitsch. f. Immunitatsforschung, i, 676. 



3 Compt. rend, de la Soc. biol., 1907. 

 Wien. Win. Woch., 1908, No. 30. 



* Ibid., No. 28. 



6 Ann. de 1'Institut Pasteur, 1907-08. 

 17 



