PROTEIN SENSITIZATION OR ANAPHYLAXIS 277 



TABLE XXIX 



No. 



1 

 2 

 3 

 4 

 5 

 6 

 7 



Effect. 



Dead in 30 minutes 

 Dead in 6 minutes 

 First and second stgaes 

 Dead in 6 minutes 

 First and second stages 

 Dead in 5 minutes 

 First stage 

 First and second stages 



It seems from this that fifteen minutes is too short a 

 time for the full development of the poison. 



7. The ferment passes through hardened filter paper. 

 The serum and organ extracts from an animal killed twenty 

 days after sensitization to egg-white were filtered through 

 hardened paper. To each 5 c.c. of the filtrates 1 ing. of 

 egg-white protein was added, incubated for thirty minutes, 

 and then injections were made intracardiacly in fresh 

 guinea-pigs, with the results shown in Table XXX. 



TABLE XXX 



No. 

 1 

 2 

 3 



4 



Fluid. 



Serum (3 c.c.) 

 Liver 

 Kidney 

 Spleen 



Effect. 



Death delayed 12 hours 

 First and second stages 

 Dead in 8 minutes 

 Dead in 6 minutes 



8. The ferment passes through a Berkefeld filter. The 

 serum and organ extracts of an animal killed twenty-two 

 days after sensitization to egg-white were filtered through 

 a Berkefeld V. To each 5 c.c. of these filtrates 1 mg. of 

 egg-white protein was added, incubated for thirty minutes, 

 and then intracardiac injections were made in fresh guinea- 

 pigs with results shown in Table XXXI. 



TABLE XXXI 



No. 

 1 

 2 

 3 



4 



Fluid. 

 Serum 

 Liver 

 Kidney 

 Spleen 



Effect. 



Dead in 6 minutes 

 Dead in 9 minutes 

 Dead in 4 minutes 

 Dead in 6 minutes 



